Work Packages

The project will last 24 months, and it is divided into 5 Work Packages (WP) with known deliverables (The resources for numerical modeling already exist at University of Cyprus (UCY) and the ones for CAP, Cytometry exist at Alexandru Ioan Cuza University at Iasi, Romania (UAIC). The cell lines, culture medium, well plates, dyes and other small items will be bought from fellowship funds. The deliverables (D) and milestones are designated (M) below.

  • WP1. Project Management
    This ensures all WPs are delivered on time (e.g. all parts are ordered, all experiments are undertaken etc.). D1.1 is the Risk Management plan. It has provisions for high-risk tasks. M1 is the discussion and acceptance by the advisory committee of this plan. D1.2 is a purchasing plan that will be done in coordination with UAIC for all items that are needed to complete this project, their price, availability, special procedures and lead-time.

  • WP2. Career Development
    The aim is the development of the Fellows career so that the Fellowship will open up new career opportunities after the Fellowship is completed.

  • WP3. Dissemination of the Results
    Engagement with the communication office of UCY (D3.1) will commence early, along with putting together Facebook, webpage with initial results (D3.2). The Fellow will also engage surgeons, entrepreneurs, by the end of the first year (D3.3).

  • WP4. Numerical Model
    Combines tasks from RO1 and RO2. First both the numerical model (D4.1) and the physical CAP source (D4.2) must be adjusted to include N2 and O2 and the numerical and experimental results are compared (D4.3). Once a validated numerical model is obtained (M5), it is extended to cell interactions (D4.4) to study the effects of electroporation and apoptosis (D4.5).

  • WP5. CAP interaction with cell lines
    In D5.1 CAP is adjusted to the conditions from D4.5 and its major electrical and optical characteristics are measured and checked good agreement to the numerical results for. Then it is applied to cells and electroporicity is quantified using cytometry (D5.2). From there the Fellow will apply CAP to cells and use cytometry to show apoptosis occurs only for cancerous cells and write the second peer-reviewed article (M7).